Reactions at the termini of tRNA with T4 RNA ligase. Journal Article uri icon

Overview

abstract

  • T4 RNA ligase will catalyze the addition of nucleoside 3', 5'-bisphosphates onto the 3' terminus of tRNA resulting in tRNA molecule one nucleotide longer with a 3' terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used, it provides an efficient means for in vitro labeling of tRNA. Although the 3' terminal hydroxyl is a good acceptor, the 5' terminal phosphate in most tRNA's is not an effective donor in the RNA ligase reaction. This poor reactivity is due to the secondary structure of the 5' terminal nucleotide. If E. Coli tRNAf Met is used, the 5' phosphate is reactive and the major product with RNA ligase is the cyclic tRNA.

publication date

  • October 1, 1978

has subject area

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Bruce AG; Uhlenbeck OC

author count

  • 2

citation count

  • 152

Other Profiles

International Standard Serial Number (ISSN)

  • 0305-1048

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Additional Document Info

start page

  • 3665

end page

  • 3677

volume

  • 5

issue

  • 10