Equimolar addition of oligoribonucleotides with T4 RNA ligase. Journal Article uri icon

Overview

abstract

  • T4 induced RNA ligase will join equimolar concentrations of two oligoribonucleotides, (Ap)3C and p(Up) 5, to form a single product, (Ap)3Cp(Up) 5, in high yield. The presence of the 3' phosphate on p(Up)5 prevents the oligomer from adding to itself. The pH optimum of the reaction is about 7.5, but less of the undesirable adenylated intermediate, App(Up) 5, forms at pH 8.2. The reaction rate is a linear function of oligomer concentration from 3 micronM to 0.6 mM. The data suggest that T4 RNA ligase will be a useful enzyme for the synthesis of oligomers of defined sequence.

publication date

  • January 1, 1977

has subject area

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Uhlenbeck OC; Cameron V

author count

  • 2

citation count

  • 32

Other Profiles

International Standard Serial Number (ISSN)

  • 0305-1048

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Additional Document Info

start page

  • 85

end page

  • 98

volume

  • 4

issue

  • 1