Role of ANC-1 in tethering nuclei to the actin cytoskeleton. Journal Article uri icon

Overview

abstract

  • Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.

publication date

  • October 1, 2002

Full Author List

  • Starr DA; Han M

Other Profiles

Additional Document Info

start page

  • 406

end page

  • 409

volume

  • 298

issue

  • 5592