Role of the core DNA polymerase III subunits at the replication fork. Alpha is the only subunit required for processive replication. Journal Article uri icon

Overview

abstract

  • The DNA polymerase III holoenzyme is composed of 10 subunits. The core of the polymerase contains the catalytic polymerase subunit, alpha, the proofreading 3'-->5' exonuclease, epsilon, and a subunit of unknown function, theta. The availability of the holoenzyme subunits in purified form has allowed us to investigate their roles at the replication fork. We show here that of the three subunits in the core polymerase, only alpha is required to form processive replication forks that move at high rates and that exhibit coupled leading- and lagging-strand synthesis in vitro. Taken together with previous data this suggests that the primary determinant of replication fork processivity is the interaction between another holoenzyme subunit, tau, and the replication fork helicase, DnaB.

publication date

  • January 1, 1998

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Marians KJ; Hiasa H; Kim DR; McHenry CS

author count

  • 4

citation count

  • 23

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9258

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

Additional Document Info

start page

  • 2452

end page

  • 2457

volume

  • 273

issue

  • 4