My main research interests are functional and structural analyses by 3-D cryo-electron microscopy of cytoskeletal assemblies and macromolecular structures as well as cellular organelles. We apply cryo-electron tomography to investigate the structure function relationships of large macromolecular assemblies and cellular organelles within their native cytosolic environment. We operate two electron microscopes with the latest data recording systems, and peripheral equipment for specimen preparation and data analysis. Our projects are the following: Giardia cytoskeleton, Large-scale supermontages of entire mammalian cells, development of high-density labels for cryo-electron microscopy, structure-function analysis of vimentin and desmin intermediate filaments, development of vitrified sections for high-resolution molecular structures in cells and tissues, and the development of software for data acquisition, tomographic, and signle-pa 3-D reconstruction, and image processing.
Cellbiology and Cytoskeleton assemblies, 3-D reconstruction of macromolecular assemblies and cellular organelles by cryo-electron microscopy, cryo-electron tomography
MCDB 3501 - Structural Methods for Biological Macromolecules
Fall 2018 / Fall 2019 / Fall 2020
Teaches fundamental knowledge about protein structures, protein interactions and protein folding. Discusses in detail the most common methods on how proteins and macromolecular complexes are studied, such as X-ray crystallography, NMR-spectroscopy and electron microscopy. Offers about 50 percent direct teaching, 40 percent discussion of papers in a journal club style and 10 percent hands-on practicals on software packages relevant to structural biology. Recommended prerequisite: MCDB 2150 or EBIO 2070 (minimum grade C-). Formerly MCDB 4501.
MCDB 4100 - Special Topics
May be repeated up to 6 total credit hours. Recommended prerequisites: MCDB 3135 and MCDB 3145 (minimum grade C-).