My main research interests are functional and structural analyses by 3-D cryo-electron microscopy of cytoskeletal assemblies and large macromolecular structures as well as cellular organelles. We apply cryo-electron tomography to investigate the structure function relationships of large macromolecular assemblies and cellular organelles within their native cytosolic environment. We operate two electron microscopes with the latest data recording systems, and peripheral equipment for specimen preparation and data analysis. Our projects are the following: Giardia cytoskeleton, Large-scale supermontages of entire mammalian cells, development of high-density labels for cryo-electron microscopy, structure-function analysis of vimentin and desmin intermediate filaments, development of vitrified sections for high-resolution molecular structures in cells and tissues, and the development of software for data acquisition, tomographic 3-D reconstruction, and image processing.
Cellbiology and Cytoskeleton assemblies, 3-D reconstruction of macromolecular assemblies and cellular organelles by cryo-electron microscopy, cryo-electron tomography