A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium. Journal Article uri icon

Overview

abstract

  • We have purified a novel DNA polymerase from Thermus thermophilus. This was enabled by use of general gap filling assays to monitor polymerase activity and cross-reactive monoclonal antibodies against the alpha catalytic subunit of E. coli DNA polymerase III holoenzyme to distinguish a novel polymerase from the well characterized DNA polymerase I-like Thermus thermophilus DNA polymerase. Two proteins migrating with the polymerase after three chromatographic steps were isolated and subjected to partial amino acid sequencing. The amino termini of both were homologous to the two products of the E. coli dnaX gene, the gamma and tau subunits of the DNA polymerase III holoenzyme. Using this information and sequences conserved among dnaX-like genes, we isolated a gene fragment by PCR and used it as a probe to isolate the full length Thermus thermophilus dnaX gene. The deduced amino acid sequence is highly homologous to the DnaX proteins of other bacteria. Examination of the sequence permitted identification of a frameshift site similar to the one used in E. coli to direct the synthesis of the shorter gamma DnaX-gene product. Based on this information, we conclude that a conventional replicase exists in extreme thermophilic eubacteria. The general biological and practical technological implications of this finding are discussed.

publication date

  • September 1, 1997

has restriction

  • closed

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • McHenry CS; Seville M; Cull MG

author count

  • 3

citation count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-2836

Electronic International Standard Serial Number (EISSN)

  • 1089-8638

Additional Document Info

start page

  • 178

end page

  • 189

volume

  • 272

issue

  • 2