B2 RNA represses TFIIH phosphorylation of RNA polymerase II. Journal Article uri icon

Overview

abstract

  • Mouse B2 RNA represses RNA polymerase II (Pol II) transcription during the cellular heat shock response. B2 RNA binds Pol II, enters complexes at promoters, and keeps the polymerase from engaging the DNA. Here we show that phosphorylation of Ser5 residues in the Pol II carboxy terminal domain (CTD) decreases after heat shock at the promoter of the repressed actin gene in mouse cells, despite the continued presence of Cdk7 and cyclin H. Biochemical assays revealed that B2 RNA, when present with Pol II in promoter-bound complexes, specifically represses CTD phosphorylation by TFIIH.

publication date

  • January 1, 2011

Full Author List

  • Yakovchuk P; Goodrich JA; Kugel JF

Other Profiles

Additional Document Info

start page

  • 45

end page

  • 49

volume

  • 2

issue

  • 1