Mutagenesis, Site-Directed PubMed MeSh Term

Overview

subject area of

• A RHO GTPase-mediated pathway is required during P cell migration in Caenorhabditis elegans  Journal Article
• A comparative study of metabolic engineering anti-metabolite tolerance in Escherichia coli  Journal Article
• A dual parameter FRET probe for measuring PKC and PKA activity in living cells  Journal Article
• A thermodynamic analysis of the sequence-specific binding of RNA by bacteriophage MS2 coat protein  Journal Article
• ALTERNATIVE SECONDARY STRUCTURES IN THE 5' EXON AFFECT BOTH FORWARD AND REVERSE SELF-SPLICING OF THE TETRAHYMENA INTERVENING SEQUENCE RNA  Journal Article
• AUTONOMOUS FOLDING AND COENZYME BINDING OF THE EXCISED PYRIDOXAL 5'-PHOSPHATE BINDING DOMAIN OF ASPARTATE-AMINOTRANSFERASE FROM ESCHERICHIA-COLI  Journal Article
• Activation of the mitogen-activated protein kinase pathway in U937 leukemic cells induces phosphorylation of the amino terminus of the TATA-binding protein  Journal Article
• Alanine scanning of MS2 coat protein reveals protein-phosphate contacts involved in thermodynamic hot spots.  Journal Article
• An In Vivo Selection Identifies Listeria monocytogenes Genes Required to Sense the Intracellular Environment and Activate Virulence Factor Expression  Journal Article
• Arg304 of human DNA primase is a key contributor to catalysis and NTP binding: Primase and the family X polymerases share significant sequence homology  Journal Article
• Attractant regulation of the aspartate receptor-kinase complex: Limited cooperative interactions between receptors and effects of the receptor modification state  Journal Article
• BINDING OF THE CBP2 PROTEIN TO A YEAST MITOCHONDRIAL GROUP-I INTRON REQUIRES THE CATALYTIC CORE OF THE RNA  Journal Article
• C2 domain of protein kinase C alpha: Elucidation of the membrane docking surface by site-directed fluorescence and spin labeling  Journal Article
• CATALYSIS OF RNA CLEAVAGE BY A RIBOZYME DERIVED FROM THE GROUP-I INTRON OF ANABAENA PRE-TRNA(LEU)  Journal Article
• Conformational changes in the photocycle of Anabaena sensory rhodopsin: absence of the Schiff base counterion protonation signal.  Journal Article
• Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching  Journal Article
• Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure  Journal Article
• Cyclophilins Facilitate Dissociation of the Human Papillomavirus Type 16 Capsid Protein L1 from the L2/DNA Complex following Virus Entry  Journal Article
• Cysteine and disulfide scanning reveals a regulatory alpha-helix in the cytoplasmic domain of the aspartate receptor  Journal Article
• DETERMINATION OF RECOGNITION NUCLEOTIDES FOR ESCHERICHIA-COLI PHENYLALANYL-TRANSFER RNA-SYNTHETASE  Journal Article
• Destabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone.  Journal Article
• Different structural changes occur in blue- and green-proteorhodopsins during the primary photoreaction.  Journal Article
• Directed mutagenesis identifies amino acid residues involved in elongation factor Tu binding to yeast Phe-tRNAPhe.  Journal Article
• Does protein structure influence trypsin miscleavage?  Journal Article
• Effect of PIP(2) binding on the membrane docking geometry of PKC alpha C2 domain: An EPR site-directed spin-labeling and relaxation study  Journal Article
• Engineered Socket Study of Signaling through a Four-Helix Bundle: Evidence for a Yin-Yang Mechanism in the Kinase Control Module of the Aspartate Receptor  Journal Article
• Engineering of a Brighter Variant of the FusionRed Fluorescent Protein Using Lifetime Flow Cytometry and Structure-Guided Mutations  Journal Article
• Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: Cysteine and disulfide scanning studies  Journal Article
• Fusogenic activity of EFF-1 is regulated via dynamic localization in fusing somatic cells of C-elegans  Journal Article
• Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution  Journal Article
• Growth and muscle defects in mice lacking adult myosin heavy chain genes  Journal Article
• His-75 in proteorhodopsin, a novel component in light-driven proton translocation by primary pumps.  Journal Article
• Human immunodeficiency virus reverse transcriptase. Expression in Escherichia coli, purification, and characterization of a functionally and structurally asymmetric dimeric polymerase.  Journal Article
• Identification of a novel cis-regulatory element involved in the heat shock response in Caenorhabditis elegans using microarray gene expression and computational methods  Journal Article
• Identification of a site critical for kinase regulation on the central processing unit (CPU) helix of the aspartate receptor  Journal Article
• Identification of novel phosphorylation sites on Xenopus laevis aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography  Journal Article
• Identification of the acidic residues in the active site of DNA polymerase III.  Journal Article
• In vivo aggregation of beta-amyloid peptide variants  Journal Article
• Increasing and Decreasing the Ultrastability of Bacterial Chemotaxis Core Signaling Complexes by Modifying Protein-Protein Contacts  Journal Article
• Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations.  Journal Article
• Interaction of the Bacillus stearothermophilus ribosomal protein S15 with 16 S rRNA .2. Specificity determinants of RNA-protein recognition  Journal Article
• Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly  Journal Article
• Interdependent domains controlling the enzymatic activity of mitogen-activated protein kinase kinase 1  Journal Article
• Intracellular calcium and myosin isoform transitions - Calcineurin and calcium-calmodulin kinase pathways regulate preferential activation of the iia myosin heavy chain promoter  Journal Article
• Joining the two domains of a group I ribozyme to form the catalytic core  Journal Article
• Kinesin's second step  Journal Article
• Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis  Journal Article
• Kinetic tuning of the EF-Hand calcium binding motif: The gateway residue independently adjusts (I) barrier height and (II) equilibrium  Journal Article
• LOCK ON OFF DISULFIDES IDENTIFY THE TRANSMEMBRANE SIGNALING HELIX OF THE ASPARTATE RECEPTOR  Journal Article
• Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A(2)  Journal Article
• MITOGEN-ACTIVATED PROTEIN (MAP) KINASE PHOSPHORYLATION OF MAP KINASE KINASE - DETERMINATION OF PHOSPHORYLATION SITES BY MASS-SPECTROMETRY AND SITE-DIRECTED MUTAGENESIS  Journal Article
• MOLECULAR GENETIC-STUDIES OF THE CDC7 PROTEIN-KINASE AND INDUCED MUTAGENESIS IN YEAST  Journal Article
• MUTATIONS AT THE GUANOSINE-BINDING SITE OF THE TETRAHYMENA RIBOZYME ALSO AFFECT SITE-SPECIFIC HYDROLYSIS  Journal Article
• MUTATIONS IN A NONCONSERVED SEQUENCE OF THE TETRAHYMENA RIBOZYME INCREASE ACTIVITY AND SPECIFICITY  Journal Article
• Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals  Journal Article
• Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation  Journal Article
• Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study  Journal Article
• Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling  Journal Article
• Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling.  Journal Article
• Microtubule-kinesin interface mutants reveal a site critical for communication  Journal Article
• Motor domain mutation traps kinesin as a microtubule rigor complex  Journal Article
• Multiplex Evolution of Antibody Fragments Utilizing a Yeast Surface Display Platform  Journal Article
• Mutually Exclusive Binding of Telomerase RNA and DNA by Ku Alters Telomerase Recruitment Model  Journal Article
• Myosin filament assembly requires a cluster of four positive residues located in the rod domain  Journal Article
• Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins  Journal Article
• OS-FRET: A New One-Sample Method for Improved FRET Measurements  Journal Article
• OXYTRICHA TELOMERE-BINDING PROTEIN - SEPARABLE DNA-BINDING AND DIMERIZATION DOMAINS OF THE ALPHA-SUBUNIT  Journal Article
• Optimizing the metal binding parameters of an EF-hand-like calcium chelation loop: Coordinating side chains play a more important tuning role than chelation loop flexibility  Journal Article
• Peptidyl-transferase ribozymes: trans reactions, structural characterization and ribosomal RNA-like features  Journal Article
• Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR  Journal Article
• Promoter scanning for transcription inhibition with DNA-binding polyamides  Journal Article
• RECOGNITION OF YEAST TRANSFER RNA(PHE) BY ITS COGNATE YEAST PHENYLALANYL-TRANSFER RNA-SYNTHETASE - AN ANALYSIS OF SPECIFICITY  Journal Article
• REGULATION OF SCALLOP MYOSIN BY MUTANT REGULATORY LIGHT-CHAINS  Journal Article
• ROLE OF THE SYK AUTOPHOSPHORYLATION SITE AND SH2 DOMAINS IN B-CELL ANTIGEN RECEPTOR SIGNALING  Journal Article
• Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors  Journal Article
• Requirement for BMP signaling in interdigital apoptosis and scale formation  Journal Article
• Role of the loop containing residue 115 in the induced-fit mechanism of the bacterial cell wall biosynthetic enzyme MurA  Journal Article
• SELF-SPLICING OF THE GROUP-I INTRON FROM ANABAENA PRE-TRANSFER-RNA - REQUIREMENT FOR BASE-PAIRING OF THE EXONS IN THE ANTICODON STEM  Journal Article
• SEQUENCE REQUIREMENTS OF THE HAMMERHEAD RNA SELF-CLEAVAGE REACTION  Journal Article
• STRUCTURE AND DYNAMICS OF ESCHERICHIA-COLI CHEMOSENSORY RECEPTORS - ENGINEERED SULFHYDRYL STUDIES  Journal Article
• Serine residues in the alpha 4 nicotinic acetylcholine receptor subunit regulate surface alpha 4 beta 2(star) receptor expression and clustering  Journal Article
• Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor  Journal Article
• Signaling domain of the aspartate receptor is a helical hairpin with a localized kinase docking surface: Cysteine and disulfide scanning studies  Journal Article
• Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function  Journal Article
• Site-directed mutagenesis reveals the thermodynamic requirements for single-stranded DNA recognition by the telomere-binding protein Cdc13  Journal Article
• Statistically Optimal Continuous Free Energy Surfaces from Biased Simulations and Multistate Reweighting  Journal Article
• Structural characterization of H3K56Q nucleosomes and nucleosomal arrays  Journal Article
• Structure of the Human cGAS-DNA Complex Reveals Enhanced Control of Immune Surveillance  Journal Article
• TELOMERIC PROTEIN-DNA POINT CONTACTS IDENTIFIED BY PHOTO-CROSS-LINKING USING 5-BROMODEOXYURIDINE  Journal Article
• THERMAL MOTIONS OF SURFACE ALPHA-HELICES IN THE D-GALACTOSE CHEMOSENSORY RECEPTOR - DETECTION BY DISULFIDE TRAPPING  Journal Article
• TRANSLATION INITIATION COMPLEX-FORMATION WITH 30-S RIBOSOMAL PARTICLES MUTATED AT CONSERVED POSITIONS IN THE 3'-MINOR DOMAIN OF 16-S RNA  Journal Article
• TRANSMEMBRANE SIGNALING BY THE ASPARTATE RECEPTOR - ENGINEERED DISULFIDES REVEAL STATIC REGIONS OF THE SUBUNIT INTERFACE  Journal Article
• Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase  Journal Article
• The 51-63 base pair of tRNA confers specificity for binding by EF-Tu.  Journal Article
• The Euplotes La motif protein p43 has properties of a telomerase-specific subunit  Journal Article
• The GRP1 PH Domain, Like the AKT1 PH Domain, Possesses a Sentry Glutamate Residue Essential for Specific Targeting to Plasma Membrane PI(3,4,5)P-3  Journal Article
• The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid.  Journal Article
• The hydroxyl of threonine 13 of the bovine 70-kDa heat shock cognate protein is essential for transducing the ATP-induced conformational change.  Journal Article
• The interface between Escherichia coli elongation factor Tu and aminoacyl-tRNA.  Journal Article
• Thermal Domain Motions of CheA Kinase in Solution: Disulfide Trapping Reveals the Motional Constraints Leading to Trans-autophosphorylation  Journal Article
• Transmission of mutant phenotypes from ES cells to adult mice  Journal Article
• Tuning the equilibrium ion affinity and selectivity of the EF-hand calcium binding motif: Substitutions at the gateway position  Journal Article
• Tyrosines in the Kinesin-5 Head Domain Are Necessary for Phosphorylation by Wee1 and for Mitotic Spindle Integrity  Journal Article
• Ubiquitination of p21(Cip1/WAF1) by SCFSkp2: Substrate requirement and ubiquitination site selection  Journal Article
• User-defined single pot mutagenesis using unamplified oligo pools  Journal Article
• p53 activates transcription by directing structural shifts in Mediator  Journal Article

broader concept

• Protein Engineering  PubMed MeSh Term