Mutagenesis, Site-Directed
PubMed MeSh Term
Overview
subject area of
-
A RHO GTPase-mediated pathway is required during P cell migration in Caenorhabditis elegans Journal Article
-
A comparative study of metabolic engineering anti-metabolite tolerance in Escherichia coli Journal Article
-
A dual parameter FRET probe for measuring PKC and PKA activity in living cells Journal Article
-
A thermodynamic analysis of the sequence-specific binding of RNA by bacteriophage MS2 coat protein Journal Article
-
ALTERNATIVE SECONDARY STRUCTURES IN THE 5' EXON AFFECT BOTH FORWARD AND REVERSE SELF-SPLICING OF THE TETRAHYMENA INTERVENING SEQUENCE RNA Journal Article
-
AUTONOMOUS FOLDING AND COENZYME BINDING OF THE EXCISED PYRIDOXAL 5'-PHOSPHATE BINDING DOMAIN OF ASPARTATE-AMINOTRANSFERASE FROM ESCHERICHIA-COLI Journal Article
-
Activation of the mitogen-activated protein kinase pathway in U937 leukemic cells induces phosphorylation of the amino terminus of the TATA-binding protein Journal Article
-
Alanine scanning of MS2 coat protein reveals protein-phosphate contacts involved in thermodynamic hot spots. Journal Article
-
An In Vivo Selection Identifies Listeria monocytogenes Genes Required to Sense the Intracellular Environment and Activate Virulence Factor Expression Journal Article
-
Arg304 of human DNA primase is a key contributor to catalysis and NTP binding: Primase and the family X polymerases share significant sequence homology Journal Article
-
Attractant regulation of the aspartate receptor-kinase complex: Limited cooperative interactions between receptors and effects of the receptor modification state Journal Article
-
BINDING OF THE CBP2 PROTEIN TO A YEAST MITOCHONDRIAL GROUP-I INTRON REQUIRES THE CATALYTIC CORE OF THE RNA Journal Article
-
C2 domain of protein kinase C alpha: Elucidation of the membrane docking surface by site-directed fluorescence and spin labeling Journal Article
-
CATALYSIS OF RNA CLEAVAGE BY A RIBOZYME DERIVED FROM THE GROUP-I INTRON OF ANABAENA PRE-TRNA(LEU) Journal Article
-
Conformational changes in the photocycle of Anabaena sensory rhodopsin: absence of the Schiff base counterion protonation signal. Journal Article
-
Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching Journal Article
-
Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure Journal Article
-
Cyclophilins Facilitate Dissociation of the Human Papillomavirus Type 16 Capsid Protein L1 from the L2/DNA Complex following Virus Entry Journal Article
-
Cysteine and disulfide scanning reveals a regulatory alpha-helix in the cytoplasmic domain of the aspartate receptor Journal Article
-
DETERMINATION OF RECOGNITION NUCLEOTIDES FOR ESCHERICHIA-COLI PHENYLALANYL-TRANSFER RNA-SYNTHETASE Journal Article
-
Destabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone. Journal Article
-
Different structural changes occur in blue- and green-proteorhodopsins during the primary photoreaction. Journal Article
-
Directed mutagenesis identifies amino acid residues involved in elongation factor Tu binding to yeast Phe-tRNAPhe. Journal Article
-
Does protein structure influence trypsin miscleavage? Journal Article
-
Effect of PIP(2) binding on the membrane docking geometry of PKC alpha C2 domain: An EPR site-directed spin-labeling and relaxation study Journal Article
-
Engineered Socket Study of Signaling through a Four-Helix Bundle: Evidence for a Yin-Yang Mechanism in the Kinase Control Module of the Aspartate Receptor Journal Article
-
Engineering of a Brighter Variant of the FusionRed Fluorescent Protein Using Lifetime Flow Cytometry and Structure-Guided Mutations Journal Article
-
Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: Cysteine and disulfide scanning studies Journal Article
-
Fusogenic activity of EFF-1 is regulated via dynamic localization in fusing somatic cells of C-elegans Journal Article
-
Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution Journal Article
-
Growth and muscle defects in mice lacking adult myosin heavy chain genes Journal Article
-
His-75 in proteorhodopsin, a novel component in light-driven proton translocation by primary pumps. Journal Article
-
Human immunodeficiency virus reverse transcriptase. Expression in Escherichia coli, purification, and characterization of a functionally and structurally asymmetric dimeric polymerase. Journal Article
-
Identification of a novel cis-regulatory element involved in the heat shock response in Caenorhabditis elegans using microarray gene expression and computational methods Journal Article
-
Identification of a site critical for kinase regulation on the central processing unit (CPU) helix of the aspartate receptor Journal Article
-
Identification of novel phosphorylation sites on Xenopus laevis aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography Journal Article
-
Identification of the acidic residues in the active site of DNA polymerase III. Journal Article
-
In vivo aggregation of beta-amyloid peptide variants Journal Article
-
Increasing and Decreasing the Ultrastability of Bacterial Chemotaxis Core Signaling Complexes by Modifying Protein-Protein Contacts Journal Article
-
Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations. Journal Article
-
Interaction of the Bacillus stearothermophilus ribosomal protein S15 with 16 S rRNA .2. Specificity determinants of RNA-protein recognition Journal Article
-
Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly Journal Article
-
Interdependent domains controlling the enzymatic activity of mitogen-activated protein kinase kinase 1 Journal Article
-
Intracellular calcium and myosin isoform transitions - Calcineurin and calcium-calmodulin kinase pathways regulate preferential activation of the iia myosin heavy chain promoter Journal Article
-
Joining the two domains of a group I ribozyme to form the catalytic core Journal Article
-
Kinesin's second step Journal Article
-
Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis Journal Article
-
Kinetic tuning of the EF-Hand calcium binding motif: The gateway residue independently adjusts (I) barrier height and (II) equilibrium Journal Article
-
LOCK ON OFF DISULFIDES IDENTIFY THE TRANSMEMBRANE SIGNALING HELIX OF THE ASPARTATE RECEPTOR Journal Article
-
Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A(2) Journal Article
-
MITOGEN-ACTIVATED PROTEIN (MAP) KINASE PHOSPHORYLATION OF MAP KINASE KINASE - DETERMINATION OF PHOSPHORYLATION SITES BY MASS-SPECTROMETRY AND SITE-DIRECTED MUTAGENESIS Journal Article
-
MOLECULAR GENETIC-STUDIES OF THE CDC7 PROTEIN-KINASE AND INDUCED MUTAGENESIS IN YEAST Journal Article
-
MUTATIONS AT THE GUANOSINE-BINDING SITE OF THE TETRAHYMENA RIBOZYME ALSO AFFECT SITE-SPECIFIC HYDROLYSIS Journal Article
-
MUTATIONS IN A NONCONSERVED SEQUENCE OF THE TETRAHYMENA RIBOZYME INCREASE ACTIVITY AND SPECIFICITY Journal Article
-
Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals Journal Article
-
Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation Journal Article
-
Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study Journal Article
-
Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling Journal Article
-
Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling. Journal Article
-
Microtubule-kinesin interface mutants reveal a site critical for communication Journal Article
-
Motor domain mutation traps kinesin as a microtubule rigor complex Journal Article
-
Multiplex Evolution of Antibody Fragments Utilizing a Yeast Surface Display Platform Journal Article
-
Mutually Exclusive Binding of Telomerase RNA and DNA by Ku Alters Telomerase Recruitment Model Journal Article
-
Myosin filament assembly requires a cluster of four positive residues located in the rod domain Journal Article
-
Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins Journal Article
-
OS-FRET: A New One-Sample Method for Improved FRET Measurements Journal Article
-
OXYTRICHA TELOMERE-BINDING PROTEIN - SEPARABLE DNA-BINDING AND DIMERIZATION DOMAINS OF THE ALPHA-SUBUNIT Journal Article
-
Optimizing the metal binding parameters of an EF-hand-like calcium chelation loop: Coordinating side chains play a more important tuning role than chelation loop flexibility Journal Article
-
Peptidyl-transferase ribozymes: trans reactions, structural characterization and ribosomal RNA-like features Journal Article
-
Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR Journal Article
-
Promoter scanning for transcription inhibition with DNA-binding polyamides Journal Article
-
RECOGNITION OF YEAST TRANSFER RNA(PHE) BY ITS COGNATE YEAST PHENYLALANYL-TRANSFER RNA-SYNTHETASE - AN ANALYSIS OF SPECIFICITY Journal Article
-
REGULATION OF SCALLOP MYOSIN BY MUTANT REGULATORY LIGHT-CHAINS Journal Article
-
ROLE OF THE SYK AUTOPHOSPHORYLATION SITE AND SH2 DOMAINS IN B-CELL ANTIGEN RECEPTOR SIGNALING Journal Article
-
Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors Journal Article
-
Requirement for BMP signaling in interdigital apoptosis and scale formation Journal Article
-
Role of the loop containing residue 115 in the induced-fit mechanism of the bacterial cell wall biosynthetic enzyme MurA Journal Article
-
SELF-SPLICING OF THE GROUP-I INTRON FROM ANABAENA PRE-TRANSFER-RNA - REQUIREMENT FOR BASE-PAIRING OF THE EXONS IN THE ANTICODON STEM Journal Article
-
SEQUENCE REQUIREMENTS OF THE HAMMERHEAD RNA SELF-CLEAVAGE REACTION Journal Article
-
STRUCTURE AND DYNAMICS OF ESCHERICHIA-COLI CHEMOSENSORY RECEPTORS - ENGINEERED SULFHYDRYL STUDIES Journal Article
-
Serine residues in the alpha 4 nicotinic acetylcholine receptor subunit regulate surface alpha 4 beta 2(star) receptor expression and clustering Journal Article
-
Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor Journal Article
-
Signaling domain of the aspartate receptor is a helical hairpin with a localized kinase docking surface: Cysteine and disulfide scanning studies Journal Article
-
Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function Journal Article
-
Site-directed mutagenesis reveals the thermodynamic requirements for single-stranded DNA recognition by the telomere-binding protein Cdc13 Journal Article
-
Statistically Optimal Continuous Free Energy Surfaces from Biased Simulations and Multistate Reweighting Journal Article
-
Structural characterization of H3K56Q nucleosomes and nucleosomal arrays Journal Article
-
Structure of the Human cGAS-DNA Complex Reveals Enhanced Control of Immune Surveillance Journal Article
-
TELOMERIC PROTEIN-DNA POINT CONTACTS IDENTIFIED BY PHOTO-CROSS-LINKING USING 5-BROMODEOXYURIDINE Journal Article
-
THERMAL MOTIONS OF SURFACE ALPHA-HELICES IN THE D-GALACTOSE CHEMOSENSORY RECEPTOR - DETECTION BY DISULFIDE TRAPPING Journal Article
-
TRANSLATION INITIATION COMPLEX-FORMATION WITH 30-S RIBOSOMAL PARTICLES MUTATED AT CONSERVED POSITIONS IN THE 3'-MINOR DOMAIN OF 16-S RNA Journal Article
-
TRANSMEMBRANE SIGNALING BY THE ASPARTATE RECEPTOR - ENGINEERED DISULFIDES REVEAL STATIC REGIONS OF THE SUBUNIT INTERFACE Journal Article
-
Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase Journal Article
-
The 51-63 base pair of tRNA confers specificity for binding by EF-Tu. Journal Article
-
The Euplotes La motif protein p43 has properties of a telomerase-specific subunit Journal Article
-
The GRP1 PH Domain, Like the AKT1 PH Domain, Possesses a Sentry Glutamate Residue Essential for Specific Targeting to Plasma Membrane PI(3,4,5)P-3 Journal Article
-
The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid. Journal Article
-
The hydroxyl of threonine 13 of the bovine 70-kDa heat shock cognate protein is essential for transducing the ATP-induced conformational change. Journal Article
-
The interface between Escherichia coli elongation factor Tu and aminoacyl-tRNA. Journal Article
-
Thermal Domain Motions of CheA Kinase in Solution: Disulfide Trapping Reveals the Motional Constraints Leading to Trans-autophosphorylation Journal Article
-
Transmission of mutant phenotypes from ES cells to adult mice Journal Article
-
Tuning the equilibrium ion affinity and selectivity of the EF-hand calcium binding motif: Substitutions at the gateway position Journal Article
-
Tyrosines in the Kinesin-5 Head Domain Are Necessary for Phosphorylation by Wee1 and for Mitotic Spindle Integrity Journal Article
-
Ubiquitination of p21(Cip1/WAF1) by SCFSkp2: Substrate requirement and ubiquitination site selection Journal Article
-
User-defined single pot mutagenesis using unamplified oligo pools Journal Article
-
p53 activates transcription by directing structural shifts in Mediator Journal Article
broader concept