One goal of my lab is to define the mechanism by which telomeres mediate chromosomal end protection and telomerase action using structural and biochemical tools. Understanding the mechanism of telomere maintenance is important in understanding the complex biological processes of cancer and aging. This has included detailed characterization of the proteins that bind the conserved single-stranded overhang at telomeres to understand both how their unique affinities and specificities are achieved as well as the biological roles for these activities. A second goal of my lab is to understand the protein interactions that mediate the activities of long non-coding RNAs and non-standard protein/RNA interactions. It has come as a huge surprise that the majority of the human genome is actively transcribed. We study the structure and function of the many proteins that lacking canonical RNA-binding domains that interact with these RNAs.
structural biology, telomere and telomerase, ssDNA, protein, long non-coding RNA and non-canonical protein, RNA interactions, transcription/RNA, molecular recognition
BCHM 4761 - Biochemistry Laboratory
Two 4-hour periods per week. Introduction to modern biochemical techniques. Topics include enzymology, spectrophotometry, electrophoresis, multi-step protein purification, recombinant DNA techniques and molecular cloning. Formerly CHEM 4761.
BCHM 5781 - Advanced General Biochemistry 2
Lect. Detailed consideration of contemporary topics in biochemistry, including protein structure (primary, secondary, tertiary, and quaternary), methods of structure determination and prediction, protein folding (kinetics, thermodynamics, denaturation, and renaturation), and protein dynamics (internal motions and methods of analysis). Formerly CHEM 5781.
BCHM 5801 - Advanced Signal Transduction and Cell Cycle Regulation
Lect. Advanced discussion of current research and literature in signal transduction, including ligands, receptors, and intracellular signaling pathways, as well as control on transcription, chromatin structure, DNA replication, mitosis, and cell cycle progression. Recommended prerequisites: CHEM 5771 and CHEM 5781 and MCDB 5210 or MCDB 5220. Formerly CHEM 5801.
MCDB 6000 - Introduction to Laboratory Methods
Introduces methodology and techniques used in biological research. Designed as a tutorial between a few students and one faculty member. Students are expected to read original research papers, discuss findings, and to plan and execute experiments in selected areas. May be repeated up to 15 total credit hours.