research overview
- My research is on chromosome ends (telomeres) and their replication by telomerase.
- Contact Info
Cech, Thomas R Distinguished Professor, Nobel Laureate and Howard Hughes Medical Institute Investigator
Positions
- Distinguished Professor, Biochemistry
- Distinguished Professor, Molecular, Cellular & Developmental Biology (MCDB)
- Institute Director, Biofrontiers Institute
Research Areas
Research
keywords
- Interdisciplinary research and education
Publications
selected publications
-
chapter
- THE RNA WORLD THIRD EDITION The Nature of Modern RNA Suggests a Prebiotic RNA World Preface. Cold Spring Harbor Monograph Series. IX-X. 2006
- The RNP World. Cold Spring Harbor Monograph Series. 309-326. 2006
- Overturning the Dogma: Catalytic RNA 2001
- Building a Catalytic Active Site Using Only RNA 1999
- Structure and Mechanism of the Large Catalytic RNAs: Group I and Group II Introns and Ribonuclease P 1999
- RNA: Getting the Message 1995
- The Origin of Life and the Value of Life 1995
- Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena 1987
- Viroids Contain Sequences Characteristic of Group I Introns 1987
- Ribosomal RNA Gene Expression in Tetrahymena: Transcription and RNA Splicing 1986
- Confrontational Dynamics Involved in RNA Self-Splicing 1986
- Mechanism of Self-Splicing of the Ribosomal RNA Precursor of Tetrahymena 1986
- Self-Splicing of the Ribosomal RNA Precursor of Tetrahymena 1983
-
conference proceeding
- TERT Promoter Mutations and Cancer. Federation proceedings. 2016
- TERT Promoter Mutations and Cancer. Federation proceedings. 2016
- MECHANISM AND STRUCTURE OF A CATALYTIC RNA MOLECULE. 91-110. 1993
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journal article
-
Regulation of histone methylation by automethylation of PRC2.
Genes and Development.
1416-1427.
2019
-
Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines.
Proceedings of the National Academy of Sciences.
18488-18497.
2019
-
C9orf72 and triplet repeat disorder RNAs: G-quadruplex formation, binding to PRC2 and implications for disease mechanisms.
RNA.
935-947.
2019
-
Fission yeast telosomes: non-canonical histone-containing chromatin structures dependent on shelterin and RNA.
Nucleic Acids Research.
8865-8875.
2018
-
A Lifelong Passion for All Things Ribonucleic.
Cell.
14-17.
2018
-
In Crystallo Selection to Establish New RNA Crystal Contacts.
Structure.
1275-+.
2018
-
Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits.
Genes and Development.
794-805.
2018
-
Dynamics of human telomerase recruitment depend on template-telomere base pairing.
Cell regulation.
869-880.
2018
-
Allele-Specific DNA Methylation and Its Interplay with Repressive Histone Marks at Promoter-Mutant TERT Genes.
Cell Reports.
3700-3707.
2017
-
Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA.
Nature Structural Biology.
1028-+.
2017
-
Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2.
eLife.
2017
-
Reconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity.
Nature Communications.
2017
-
How do lncRNAs regulate transcription?.
Science Advances.
2017
-
Targeting of Polycomb Repressive Complex 2 to RNA by Short Repeats of Consecutive Guanines.
Molecular Cell.
1056-+.
2017
-
Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.
Genes and Development.
59-71.
2017
-
Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres.
Cell.
1188-+.
2016
-
RNA Duplex Map in Living Cells Reveals Higher-Order Transcriptome Structure.
Cell.
1267-1279.
2016
-
Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects.
Nature Structural Biology.
286-292.
2016
-
The recruitment of chromatin modifiers by long noncoding RNAs: lessons from PRC2.
RNA.
2007-2022.
2015
-
A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression.
Genome Biology.
2015
-
Mutation of the TERT promoter, switch to active chromatin, and monoallelic TERT expression in multiple cancers.
Genes and Development.
2219-2224.
2015
-
Protein-RNA interaction restricts telomerase from running through the stop sign.
Nature Structural Biology.
835-836.
2015
-
Nucleic acid-binding specificity of human FUS protein.
Nucleic Acids Research.
7535-7543.
2015
-
Human telomerase: biogenesis, trafficking, recruitment, and activation.
Genes and Development.
1095-1105.
2015
-
RNA World research-still evolving.
RNA.
474-475.
2015
-
Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase.
Journal of Molecular Biology.
1291-1303.
2015
- Placental Expression of the lncRNA NEAT1 Increases in Preeclampsia. Journal of the Society for Gynecologic Investigation. 201A-201A. 2015
- Placental Expression of the lncRNA NEAT1, and Not MENb or TncRNA, Increases With Gestation. Journal of the Society for Gynecologic Investigation. 189A-189A. 2015
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TERT promoter mutations and telomerase reactivation in urothelial cancer.
Science.
1006-1010.
2015
-
Toward a Consensus on the Binding Specificity and Promiscuity of PRC2 for RNA.
Molecular Cell.
552-558.
2015
-
Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance.
Journal of Cell Science.
331-341.
2015
-
Biochemical Properties and Biological Functions of FET Proteins.
Annual Review of Biochemistry.
355-379.
2015
-
Companies on campus.
Nature: New biology.
297-298.
2014
-
Academia and industry: Companies on campus..
Nature: New biology.
297-298.
2014
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Identification of human TERT elements necessary for telomerase recruitment to telomeres.
eLife.
2014
-
FUS is sequestered in nuclear aggregates in ALS patient fibroblasts.
Cell regulation.
2571-2578.
2014
-
Structure and Function of Steroid Receptor RNA Activator Protein, the Proposed Partner of SRA Noncoding RNA.
Journal of Molecular Biology.
1766-1785.
2014
-
The Noncoding RNA Revolution-Trashing Old Rules to Forge New Ones.
Cell.
77-94.
2014
-
Telomerase in Bladder Cancer: Back to a Better Future?.
European Urology.
370-371.
2014
-
Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT.
Nucleic Acids Research.
8565-8577.
2014
-
NAR Breakthrough Article A dimeric state for PRC2.
Nucleic Acids Research.
9236-9248.
2014
-
Inhibition of Telomerase Recruitment and Cancer Cell Death.
Journal of Biological Chemistry.
33171-33180.
2013
-
Promiscuous RNA binding by Polycomb repressive complex 2.
Nature Structural Biology.
1250-U273.
2013
-
RNA Seeds Higher-Order Assembly of FUS Protein.
Cell Reports.
918-925.
2013
-
Many disease-associated variants of hTERT retain high telomerase enzymatic activity.
Nucleic Acids Research.
8969-8978.
2013
-
3 ' terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing.
BMC Molecular Biology.
2013
-
RNA recognition by the DNA end-binding Ku heterodimer.
RNA.
841-851.
2013
-
Finding the end: recruitment of telomerase to telomeres.
Nature Reviews Molecular Cell Biology.
71-+.
2013
-
How a Chemist Looks at RNA.
Angewandte Chemie.
75-78.
2013
-
FUS binds the CTD of RNA polymerase II and regulates its phosphorylation at Ser2.
Genes and Development.
2690-2695.
2012
-
The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity.
Nature: New biology.
285-+.
2012
-
SnapShot: Telomeres and Telomerase.
Cell.
2012
-
The RNA Worlds in Context.
Cold Spring Harbor perspectives in biology.
2012
-
RNase P branches out from RNP to protein: organelle-triggered diversification?.
Genes and Development.
1005-1009.
2012
-
Mutually Exclusive Binding of Telomerase RNA and DNA by Ku Alters Telomerase Recruitment Model.
Cell.
922-932.
2012
- Mutually exclusive binding of telomerase RNA and DNA by Ku alters telomerase recruitment model.. Cell. 922-32-922-32. 2012
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DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.
Nucleic Acids Research.
235-244.
2012
- DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.. 235-44-235-44. 2012
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The RNA accordion model for template positioning by telomerase RNA during telomeric DNA synthesis.
Nature Structural Biology.
1371-U84.
2011
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Multiple POT1-TPP1 Proteins Coat and Compact Long Telomeric Single-Stranded DNA.
Journal of Molecular Biology.
10-17.
2011
-
Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells.
Nature: New biology.
399-+.
2011
-
Ku can contribute to telomere lengthening in yeast at multiple positions in the telomerase RNP.
RNA.
298-311.
2011
-
Tetrahymena Telomerase Protein p65 Induces Conformational Changes throughout Telomerase RNA (TER) and Rescues Telomerase Reverse Transcriptase and TER Assembly Mutants.
Molecular and Cellular Biology.
4965-4976.
2010
-
Engineering cis-telomerase RNAs that add telomeric repeats to themselves.
Proceedings of the National Academy of Sciences.
4914-4918.
2010
-
Functional interaction between telomere protein TPP1 and telomerase.
Genes and Development.
613-622.
2010
-
POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation.
EMBO Journal.
924-933.
2010
-
How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.
Proceedings of the National Academy of Sciences.
651-656.
2010
-
Crawling Out of the RNA World.
Cell.
599-602.
2009
-
A natural ribozyme with 3 ',5 ' RNA ligase activity.
Nature Chemical Biology.
97-99.
2009
-
Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p.
Nucleic Acids Research.
354-367.
2009
-
Evolution of biological catalysis: ribozyme to RNP enzyme..
Cold Spring Harbor Symposia on Quantitative Biology.
11-16.
2009
-
Toward predicting self-splicing and protein-facilitated splicing of group I introns.
RNA.
2013-2029.
2008
-
Mutation in TERT separates processivity from anchor-site function.
Nature Structural Biology.
871-872.
2008
-
Multiple yeast genes, including Paf1 complex genes, affect telomere length via telomerase RNA abundance.
Molecular and Cellular Biology.
4152-4161.
2008
-
Triple-helix structure in telomerase RNA contributes to catalysis.
Nature Structural Biology.
634-640.
2008
-
On the occasion of the 25(th) anniversary of the discovery of catalytic RNA.
Biological Chemistry.
661-662.
2007
-
Local RNA structural changes induced by crystallization are revealed by SHAPE.
RNA.
536-548.
2007
-
The POT1-TPP1 telomere complex is a telomerase processivity factor.
Nature: New biology.
506-510.
2007
-
Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency.
RNA.
1721-1737.
2006
- Crystal structure of an essential telomerase-specific domain of TERT. Federation proceedings. A984-A984. 2006
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Comparison of crystal structure interactions and thermodynamics for stabilizing mutations in the Tetrahymena ribozyme.
RNA.
387-395.
2006
-
Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase.
Nature Structural Biology.
218-225.
2006
-
Atomic level architecture of group I introns revealed.
Trends in Biochemical Sciences.
41-51.
2006
-
RNA as a flexible scaffold for proteins: Yeast telomerase and beyond.
Cold Spring Harbor Symposia on Quantitative Biology.
217-224.
2006
-
A miniature yeast telomerase RNA functions in vivo and reconstitutes activity in vitro.
Nature Structural Biology.
1072-1077.
2005
-
POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates.
Journal of Biological Chemistry.
32069-32080.
2005
-
Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro.
Proceedings of the National Academy of Sciences.
10864-10869.
2005
-
Soluble domains of telomerase reverse transcriptase identified by high-throughput screening.
Protein Science.
2051-2058.
2005
-
Switching human telomerase on and off with hPOT1 protein in vitro.
Journal of Biological Chemistry.
20449-20456.
2005
-
Expression of a RecQ helicase homolog affects progression through crisis in fission yeast lacking telomerase.
Journal of Biological Chemistry.
5249-5257.
2005
- Fostering innovation and discovery in biomedical research.. 1390-3-1390-3. 2005
-
Global expression changes resulting from loss of telomeric DNA in fission yeast.
Genome Biology.
2005
-
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.
Nature Structural Biology.
1223-1229.
2004
-
Structure of the Tetrahymena ribozyme: Base triple sandwich and metal ion at the active site.
Molecular Cell.
351-362.
2004
-
Self-splicing and enzymatic activity of an intervening sequence RNA from Tetrahymena.
Bioscience Reports.
362-385.
2004
-
Yeast telomerase RNA: A flexible scaffold for protein subunits.
Proceedings of the National Academy of Sciences.
10024-10029.
2004
-
The Euplotes telomerase subunit p43 stimulates enzymatic activity and processivity in vitro.
RNA.
1108-1118.
2004
-
RNA finds a simpler way.
Nature: New biology.
263-264.
2004
-
Beginning to understand the end of the chromosome.
Cell.
273-279.
2004
- Nurturing interdisciplinary research.. 1166-9-1166-9. 2004
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Tetrahymena telomerase is active as a monomer.
Cell regulation.
4794-4804.
2003
-
DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.
Nature: New biology.
198-203.
2003
-
A template-proximal RNA paired element contributes to Saccharomyces cerevisiae telomerase activity.
RNA.
1323-1332.
2003
-
DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA..
Nature: New biology.
198-203.
2003
-
Sequence-specific and 3 '-end selective single-strand DNA binding by the Oxytricha nova telomere end binding protein alpha subunit.
Biochemistry.
9269-9277.
2003
-
Human POT1 facilitates telomere elongation by telomerase.
Current Biology.
942-946.
2003
-
The Euplotes La motif protein p43 has properties of a telomerase-specific subunit.
Biochemistry.
5736-5747.
2003
-
Yeast telomerase is specialized for C/A-rich RNA templates.
Nucleic Acids Research.
1646-1655.
2003
-
Rebalancing teaching and research.
Science.
165-165.
2003
-
N-terminal domain of yeast telomerase reverse transcriptase: Recruitment of Est3p to the telomerase complex.
Cell regulation.
1-13.
2003
- Sharing publication-related data and materials: responsibilities of authorship in the life sciences.. 19-24-19-24. 2003
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Cooperative binding of single-stranded telomeric DNA by the Pot1 protein of Schizosaccharomyces pombe.
Biochemistry.
14560-14568.
2002
-
A bulged stem tethers Est1p to telomerase RNA in budding yeast.
Genes and Development.
2800-2812.
2002
-
Evolution of Tetrahymena ribozyme mutants with increased structural stability.
Nature Structural Biology.
855-861.
2002
-
Human Pot1 (Protection of telomeres) protein: Cytolocalization, gene structure, and alternative splicing.
Molecular and Cellular Biology.
8079-8087.
2002
-
Ribozymes, the first 20 years.
Biochemical Society Transactions.
1162-1166.
2002
-
The chemical repertoire of natural ribozymes.
Nature: New biology.
222-228.
2002
-
In vivo selection of better self-splicing introns in Escherichia coli: The role of the P1 extension helix of the Tetrahymena intron.
RNA.
647-658.
2002
-
Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction..
Molecular and Cellular Biology.
2366-2374.
2002
-
Effects of nucleotide analogues on Euplotes aediculatus telomerase processivity: Evidence for product-assisted translocation.
Biochemistry.
151-161.
2002
-
A stem-loop of Tetrahymena telomerase RNA distant from the template potentiates RNA folding and telomerase activity.
Biochemistry.
7005-7016.
2001
-
Pot1, the putative telomere end-binding protein in fission yeast and humans.
Science.
1171-1175.
2001
-
Engineering disulfide cross-links in RNA using thiol-disulfide interchange chemistry..
Current Protocols in Nucleic Acid Chemistry.
Unit-5.1.
2001
-
Structural basis of the enhanced stability of a mutant ribozyme domain and a detailed view of RNA-solvent interactions.
Structure.
221-231.
2001
- Two RNA machines: Ribozyme and telomerase. Federation proceedings. A57-A57. 2001
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An early transition state for folding of the P4-P6 RNA domain.
RNA.
161-166.
2001
- Engineering disulfide cross-links in RNA using thiol-disulfide interchange chemistry.. Unit 5.1-Unit 5.1. 2001
- Science and business. Conflicts of interest–moving beyond disclosure.. Science. 989-989. 2001
-
Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting.
EMBO Journal.
6230-6239.
2000
-
Multiple folding pathways for the P4-P6 RNA domain.
Biochemistry.
12465-12475.
2000
-
Protection of telomeres by the Ku protein in fission yeast.
Cell regulation.
3265-3275.
2000
-
A mutant of Tetrahymena telomerase reverse transcriptase with increased processivity.
Journal of Biological Chemistry.
24199-24207.
2000
-
Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase.
Molecular Cell.
493-499.
2000
-
Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomyces pombe.
Proceedings of the National Academy of Sciences.
6367-6372.
2000
-
Life at the end of the chromosome: Telomeres and telomerase.
Angewandte Chemie.
34-43.
2000
- Structural biology. The ribosome is a ribozyme.. Science. 878-9-878-9. 2000
-
Quantifying the energetic interplay of RNA tertiary and secondary structure interactions.
RNA.
1665-1674.
1999
- Science at liberal arts colleges: A better education?. Daedalus. 195-216. 1999
-
Self-splicing of the Tetrahymena intron from mRNA in mammalian cells.
EMBO Journal.
6491-6500.
1999
-
Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants.
Genes and Development.
2863-2874.
1999
-
RNA tertiary folding monitored by fluorescence of covalently attached pyrene.
Biochemistry.
14224-14237.
1999
- Hot papers - Telomerase - Telomerase catalytic subunit homologs from fission yeast and human by T.M. Nakamura, G.B. Morin, K.B. Chapman, S.L. Weinrich, W.H. Andrews, J. Lingner, C.B. Harley, T.E. Cech - Comments. Scientist -Philadelphia-. 14-14. 1999
- Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle. Nature: New biology. 177-180. 1999
-
In vitro selection of RNAs with increased tertiary structure stability.
RNA.
1119-1129.
1999
-
Energetics and cooperativity of tertiary hydrogen bonds in RNA structure.
Biochemistry.
8691-8702.
1999
-
Telomerase and the maintenance of chromosome ends.
Current Opinion in Cell Biology.
318-324.
1999
-
In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.
RNA.
1481-1492.
1998
-
Two modes of survival of fission yeast without telomerase.
Science.
493-496.
1998
-
A preorganized active site in the crystal structure of the Tetrahymena ribozyme.
Science.
259-264.
1998
-
A quantitative study of the flexibility contributed to RNA structures by nicks and single-stranded gaps.
RNA.
1179-1185.
1998
-
Peptidyl-transferase ribozymes: trans reactions, structural characterization and ribosomal RNA-like features.
Cell Chemistry Biology.
539-553.
1998
-
Thermodynamic stability of the P4-P6 domain RNA tertiary structure measured by temperature gradient gel electrophoresis.
Biochemistry.
11162-11170.
1998
-
Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax.
Proceedings of the National Academy of Sciences.
8479-8484.
1998
-
Modulation of telomerase activity by telomere DNA-binding proteins in Oxytricha.
Genes and Development.
1504-1514.
1998
-
Binding of the protein kinase PKR to RNAs with secondary structure defects: Role of the tandem A-G mismatch and noncontiguous helixes.
Biochemistry.
6303-6316.
1998
-
Euplotes telomerase: Evidence for limited base-pairing during primer elongation and dGTP as an effector of translocation.
Biochemistry.
5162-5172.
1998
-
Telomerase and chromosome end maintenance.
Current Opinion in Genetics and Development.
226-232.
1998
-
Reversing time: Origin of telomerase.
Cell.
587-590.
1998
- Are telomeres correlative or causative in cellular senescence? General discussion. Ciba Foundation symposium. 145-147. 1998
- Human ageing and telomeres - Discussion. Ciba Foundation symposium. 139-144. 1998
- Mouse models for the study of telomerase - Discussion. Ciba Foundation symposium. 170-176. 1998
- Repair and processing events at DNA ends - Discussion. Ciba Foundation symposium. 205-208. 1998
- Telomerase and the chromosome end replication problem. Ciba Foundation symposium. 20-28. 1998
- Telomerase assays in the diagnosis and prognosis of cancer - Discussion. Ciba Foundation symposium. 155-159. 1998
- Telomeres and telomerase in other organisms. Ciba Foundation symposium. 223-226. 1998
- Telomeres in the haemopoietic system - Discussion. Ciba Foundation symposium. 219-222. 1998
-
Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis.
Biochemistry.
16345-16354.
1997
-
Peptide bond formation by in vitro selected ribozymes.
Nature: New biology.
96-100.
1997
- Polyadenylation of telomerase RNA in budding yeast. RNA. 1337-1351. 1997
- Telomerase is a true reverse transcriptase. A review. Biokhimiia (Moscow, Russia). 1202-1205. 1997
-
Three ever shorter telomere (EST) genes are dispensable for in vitro yeast telomerase activity.
Proceedings of the National Academy of Sciences.
11190-11195.
1997
-
dGTP-dependent processivity and possible template switching of Euplotes telomerase.
Nucleic Acids Research.
3698-3704.
1997
- Mutagenesis and comparative sequence analysis of a base triple joining the two domains of group I ribozymes. RNA. 1037-1051. 1997
-
A second catalytic metal ion in a group I ribozyme.
Nature: New biology.
805-808.
1997
-
Telomerase catalytic subunit homologs from fission yeast and human.
Science.
955-959.
1997
- An RNA internal loop acts as a hinge to facilitate ribozyme folding and catalysis. RNA. 838-849. 1997
-
Crystals by design: A strategy for crystallization of a ribozyme derived from the Tetrahymena group I intron.
Journal of Molecular Biology.
711-723.
1997
-
Dynamics of thermal motions within a large catalytic RNA investigated by cross-linking with thiol-disulfide interchange.
Journal of the American Chemical Society.
6259-6268.
1997
-
Effects of divalent metal ions on individual steps of the Tetrahymena ribozyme reaction.
Biochemistry.
8293-8303.
1997
-
Reverse transcriptase motifs in the catalytic subunit of telomerase.
Science.
561-567.
1997
-
Regulation of telomere length and function by a Myb-domain protein in fission yeast.
Nature: New biology.
744-747.
1997
-
Joining the two domains of a group I ribozyme to form the catalytic core.
Science.
847-849.
1997
-
The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers.
Molecular and Cellular Biology.
296-308.
1997
- X-ray crystallography of large RNAs: Heavy-atom derivatives by RNA engineering. RNA. 1295-1305. 1996
-
The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena.
Genes and Development.
2870-2880.
1996
-
Synthesis and characterization of an RNA dinucleotide containing a 3'-S-phosphorothiolate linkage.
Journal of the American Chemical Society.
10341-10350.
1996
-
Conserved thermochemistry of guanosine nucleophile binding for structurally distinct group I ribozymes.
Nucleic Acids Research.
3722-3727.
1996
-
Purification of telomerase from Euplotes aediculatus: Requirement of a primer 3' overhang.
Proceedings of the National Academy of Sciences.
10712-10717.
1996
-
Crystal structure of a group I ribozyme domain: Principles of RNA packing.
Science.
1678-1685.
1996
- RNA tertiary structure mediation by adenosine platforms. Science. 1696-1699. 1996
-
Mutations in the Tetrahymena ribozyme internal guide sequence: Effects on docking of the P1 helix into the catalytic core and correlation with catalytic activity.
Biochemistry.
11493-11502.
1996
-
Minor-groove recognition of double-stranded RNA by the double-stranded RNA-binding domain from the RNA-activated protein kinase PKR.
Biochemistry.
9983-9994.
1996
- Kinetic pathway for folding of the Tetrahymena ribozyme revealed by three UV-inducible crosslinks. RNA. 718-732. 1996
-
Mitochondrial import of only one of three nuclear-encoded glutamine tRNAs in Tetrahymena thermophila.
EMBO Journal.
3286-3295.
1996
- Selecting apt RNAs for NMR. RNA. 625-627. 1996
-
Conformational switches involved in orchestrating the successive steps of group I RNA splicing.
Biochemistry.
3754-3763.
1996
-
Method for determining RNA 3' ends and application to human telomerase RNA.
Nucleic Acids Research.
532-533.
1996
-
Exocyclic amine of the conserved G center dot U pair at the cleavage site of the Tetrahymena ribozyme contributes to 5'-splice site selection and transition state stabilization.
Biochemistry.
1201-1211.
1996
-
Assembly of a ribonucleoprotein catalyst by tertiary structure capture.
Science.
345-348.
1996
- Activity and thermostability of the small self-splicing group I intron in the pre-tRNA(IIe) of the purple bacterium Azoarcus. RNA. 74-83. 1996
-
RELATIVE ORIENTATION OF RNA HELICES IN A GROUP-I RIBOZYME DETERMINED BY HELIX EXTENSION ELECTRON-MICROSCOPY.
EMBO Journal.
4849-4859.
1995
-
TELOMERASE AND DNA END REPLICATION - NO LONGER A LAGGING-STRAND PROBLEM.
Science.
1533-1534.
1995
- IDENTIFICATION OF RIBOZYMES WITHIN A RIBOZYME LIBRARY THAT EFFICIENTLY CLEAVE A LONG SUBSTRATE RNA. RNA. 598-609. 1995
-
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1985
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INTERMOLECULAR EXON LIGATION OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA - OLIGONUCLEOTIDES CAN FUNCTION AS 5' EXONS.
Cell.
431-437.
1985
-
OLIGOMERIZATION OF INTERVENING SEQUENCE RNA MOLECULES IN THE ABSENCE OF PROTEINS.
Science.
1060-1064.
1985
-
REACTIONS OF THE INTERVENING SEQUENCE OF THE TETRAHYMENA RIBOSOMAL RIBONUCLEIC-ACID PRECURSOR - PH-DEPENDENCE OF CYCLIZATION AND SITE-SPECIFIC HYDROLYSIS.
Biochemistry.
6211-6218.
1985
-
REVERSIBILITY OF CYCLIZATION OF THE TETRAHYMENA RIBOSOMAL-RNA INTERVENING SEQUENCE - IMPLICATION FOR THE MECHANISM OF SPLICE SITE CHOICE.
Cell.
639-648.
1985
-
SECONDARY STRUCTURE OF THE CIRCULAR FORM OF THE TETRAHYMENA RIBOSOMAL-RNA INTERVENING SEQUENCE - A TECHNIQUE FOR RNA STRUCTURE-ANALYSIS USING CHEMICAL PROBES AND REVERSE-TRANSCRIPTASE.
Proceedings of the National Academy of Sciences.
648-652.
1985
-
SELF-CATALYZED CYCLIZATION OF THE INTERVENING SEQUENCE RNA OF TETRAHYMENA - INHIBITION BY INTERCALATING DYES.
Nucleic Acids Research.
7741-7758.
1985
-
SELF-CATALYZED CYCLIZATION OF THE INTERVENING SEQUENCE RNA OF TETRAHYMENA - INHIBITION BY METHIDIUMPROPYL.EDTA AND LOCALIZATION OF THE MAJOR DYE BINDING-SITES.
Nucleic Acids Research.
7759-7779.
1985
- SELF-SPLICING OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA. Federation proceedings. R10-R10. 1985
- SELF-SPLICING RNA. ACS National Meeting Book of Abstracts. 85-BIL. 1985
- SELF-SPLICING RNA. Biochemistry. 3370-3371. 1985
-
SELF-SPLICING RNA - IMPLICATIONS FOR EVOLUTION.
International Review of Cytology.
3-22.
1985
-
SEQUENCE REQUIREMENTS FOR SELF-SPLICING OF THE TETRAHYMENA-THERMOPHILA PRE-RIBOSOMAL RNA.
Nucleic Acids Research.
1871-1889.
1985
-
A LABILE PHOSPHODIESTER BOND AT THE LIGATION JUNCTION IN A CIRCULAR INTERVENING SEQUENCE RNA.
Science.
574-578.
1984
-
CHROMATIN STRUCTURE AT THE REPLICATION ORIGINS AND TRANSCRIPTION-INITIATION REGIONS OF THE RIBOSOMAL-RNA GENES OF TETRAHYMENA.
Cell.
933-942.
1984
-
CHROMATIN STRUCTURE OF THE MOLECULAR ENDS OF OXYTRICHA MACRONUCLEAR DNA - PHASED NUCLEOSOMES AND A TELOMERIC COMPLEX.
Cell.
501-510.
1984
-
DNA - PROTEIN INTERACTIONS IN THE DROSOPHILA-VIRILIS MITOCHONDRIAL CHROMOSOME.
Nucleic Acids Research.
1991-1999.
1984
-
SPECIFIC INTERACTION BETWEEN THE SELF-SPLICING RNA OF TETRAHYMENA AND ITS GUANOSINE SUBSTRATE - IMPLICATIONS FOR BIOLOGICAL CATALYSIS BY RNA.
Nature: New biology.
820-826.
1984
-
AUTOCATALYTIC CYCLIZATION OF AN EXCISED INTERVENING SEQUENCE RNA IS A CLEAVAGE LIGATION REACTION.
Nature: New biology.
578-583.
1983
-
DIFFERENT NUCLEOSOME SPACING IN TRANSCRIBED AND NON-TRANSCRIBED REGIONS OF THE RIBOSOMAL-RNA GENE IN TETRAHYMENA-THERMOPHILA.
Nucleic Acids Research.
2093-2109.
1983
-
FATE OF AN INTERVENING SEQUENCE RIBONUCLEIC-ACID - EXCISION AND CYCLIZATION OF THE TETRAHYMENA RIBOSOMAL RIBONUCLEIC-ACID INTERVENING SEQUENCE INVIVO.
Biochemistry.
2390-2397.
1983
-
RNA SPLICING - 3 THEMES WITH VARIATIONS.
Cell.
713-716.
1983
- SECONDARY STRUCTURE OF A SELF-SPLICING RNA. Federation proceedings. 1884-1884. 1983
-
SECONDARY STRUCTURE OF THE TETRAHYMENA RIBOSOMAL-RNA INTERVENING SEQUENCE - STRUCTURAL HOMOLOGY WITH FUNGAL MITOCHONDRIAL INTERVENING SEQUENCES.
Proceedings of the National Academy of Sciences.
3903-3907.
1983
-
TRANSCRIBED AND NON-TRANSCRIBED REGIONS OF TETRAHYMENA RIBOSOMAL GENE CHROMATIN HAVE DIFFERENT ACCESSIBILITIES TO MICROCOCCAL NUCLEASE.
Nucleic Acids Research.
2077-2091.
1983
-
SELF-SPLICING RNA - AUTO-EXCISION AND AUTO-CYCLIZATION OF THE RIBOSOMAL-RNA INTERVENING SEQUENCE OF TETRAHYMENA.
Cell.
147-157.
1982
-
THE INTERVENING SEQUENCE EXCISED FROM THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA CONTAINS A 5'-TERMINAL GUANOSINE RESIDUE NOT ENCODED BY THE DNA.
Nucleic Acids Research.
2823-2838.
1982
- TRANSCRIBED AND NON-TRANSCRIBED REGIONS OF THE RIBOSOMAL-RNA GENE OF TETRAHYMENA EXHIBIT DIFFERENT CHROMATIN STRUCTURES. Federation proceedings. 514-514. 1982
- TRANSCRIPTION AND SPLICING OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA. 171-204. 1982
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ALKALINE GEL-ELECTROPHORESIS OF DEOXYRIBONUCLEIC-ACID PHOTOREACTED WITH TRIMETHYLPSORALEN - RAPID AND SENSITIVE DETECTION OF INTERSTRAND CROSS-LINKS.
Biochemistry.
1431-1437.
1981
-
INVITRO SPLICING OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA - INVOLVEMENT OF A GUANOSINE NUCLEOTIDE IN THE EXCISION OF THE INTERVENING SEQUENCE.
Cell.
487-496.
1981
-
INVITRO SPLICING OF THE RIBOSOMAL-RNA PRECURSOR OF TETRAHYMENA - INVOLVEMENT OF A GUANOSINE NUCLEOTIDE IN THE EXCISION OF THE INTERVENING SEQUENCE.
Cell.
487-496.
1981
-
REPLICATION OF THE EXTRACHROMOSOMAL RIBOSOMAL-RNA GENES OF TETRAHYMENA-THERMOPHILIA.
Nucleic Acids Research.
3531-3543.
1981
- SPLICING OF THE RIBOSOMAL-RNA PRECURSOR AND CYCLIZATION OF THE EXCISED INTERVENING SEQUENCE RNA IN NUCLEI OF TETRAHYMENA. Federation proceedings. 1651-1651. 1981
-
THE INTERVENING SEQUENCE OF THE RIBOSOMAL-RNA PRECURSOR IS CONVERTED TO A CIRCULAR RNA IN ISOLATED-NUCLEI OF TETRAHYMENA.
Cell.
467-476.
1981
-
CHROMATIN STRUCTURE OF THE RIBOSOMAL-RNA GENES OF TETRAHYMENA-THERMOPHILA AS ANALYZED BY TRIMETHYLPSORALEN CROSSLINKING INVIVO.
Journal of Molecular Biology.
395-416.
1980
-
DNA-PROTEIN INTERACTIONS IN THE DROSOPHILA-MELANOGASTER MITOCHONDRIAL GENOME AS DEDUCED FROM TRIMETHYLPSORALEN CROSSLINKING PATTERNS.
Proceedings of the National Academy of Sciences.
4118-4122.
1980
-
INVITRO SPLICING OF THE RIBOSOMAL-RNA PRECURSOR IN NUCLEI OF TETRAHYMENA.
Cell.
331-338.
1980
-
An electron microscopic study of the photochemical cross-linking of DNA in guinea pig epidermis by psoralen derivatives.
Biochimica et Biophysica Acta - Section Nucleic Acids And Protein Synthesis.
342-360.
1979
-
LOCALIZATION OF TRANSCRIBED REGIONS ON EXTRACHROMOSOMAL RIBOSOMAL-RNA GENES OF TETRAHYMENA-THERMOPHILA BY R-LOOP MAPPING.
Proceedings of the National Academy of Sciences.
5051-5055.
1979
- Chromatin structure in living cells.. 191-8-191-8. 1978
-
Electron microscopy of DNA crosslinked with trimethylpsoralen: a probe for chromatin structure.
Biochemistry.
5313-5321.
1977
-
Cross-linking of DNA with trimethylpsoralen is a probe for chromatin structure.
Cell.
631-640.
1977
- Electron microscopy of DNA crosslinked with trimethylpsoralen: test of the secondary structure of eukaryotic inverted repeat sequences.. 2644-8-2644-8. 1976
-
ORGANIZATION OF HIGHLY REPEATED SEQUENCES IN MOUSE MAIN-BAND DNA.
Journal of Molecular Biology.
227-256.
1976
-
PARTIAL DENATURATION OF MOUSE DNA IN PREPARATIVE CSCL DENSITY GRADIENTS AT ALKALINE PH.
Biochemistry.
1865-1873.
1976
-
STUDY OF DNA DENATURATION IN ULTRACENTRIFUGE.
Biopolymers.
1591-1613.
1976
-
ELECTRON-MICROSCOPIC STUDY OF MOUSE FOLDBACK DNA.
Cell.
429-446.
1975
- Characterization of the rapidly renaturing sequences in the main CsCl density bands of Drosophila, mouse, and human DNA.. 329-39-329-39. 1974
- Characterization of the most rapidly renaturing sequences in mouse main-band DNA.. 299-325-299-325. 1973
-
Regulation of histone methylation by automethylation of PRC2.
Genes and Development.
1416-1427.
2019
Teaching
courses taught
-
BCHM 4312 - Quantitative Optical Imaging
Primary Instructor - Fall 2019
Explores the fundamentals of optical imaging in biology. Covered topics include an introduction to optics and microscopes, fluorescence microscopy and image analysis. MATLAB will be taught throughout the course and used for image processing. Same as MCDB 4312, MCDB 5312 and BCHM 5312. -
BCHM 5312 - Quantitative Optical Imaging
Primary Instructor - Fall 2019
Explores the fundamentals of optical imaging in biology. Covered topics include an introduction to optics and microscopes, fluorescence microscopy and image analysis. MATLAB will be taught throughout the course and used for image processing. Same as MCDB 5312, MCDB 4312 and BCHM 4312. -
BCHM 6901 - Research in Biochemistry
Primary Instructor - Fall 2019 / Spring 2020
Research in Biochemistry -
CHEM 6901 - Research in Chemistry
Primary Instructor - Spring 2018 / Summer 2018 / Fall 2018 / Spring 2019 / Summer 2019 / Spring 2020
May be repeated up to 15 total credit hours. -
MCDB 4312 - Quantitative Optical Imaging
Primary Instructor - Fall 2018 / Fall 2019
Explores the fundamentals of optical imaging in biology. Covered topics include an introduction to optics and microscopes, fluorescence microscopy and image analysis. MATLAB will be taught throughout the course and used for image processing. Same as BCHM 4312, MCDB 5312 and BCHM 5312. -
MCDB 5312 - Quantitative Optical Imaging
Primary Instructor - Fall 2018 / Fall 2019
Explores the fundamentals of optical imaging in biology. Covered topics include an introduction to optics and microscopes, fluorescence microscopy and image analysis. MATLAB will be taught throughout the course and used for image processing. Same as BCHM 5312, MCDB 4312 and BCHM 4312.
Background
education and training
- Ph.D., University of California-Berkeley 1975
- B.A., Grinnell College 1970
awards and honors
International Activities
global connections related to teaching and scholarly work (in recent years)
- Australia Country
- Belgium Country
- Czech Republic Country
- Germany Country
- Ireland Country
- Japan Country
- Poland Country
- Singapore Country
- Switzerland Country
- United Kingdom Country